DAPI (4',6-diamidino-2-phenylindole) | Thermo Fisher ...
DAPI is generally used to stain fixed cells since the dye is cell impermeant, although the stain will enter live cells when used at higher concentrations. For live-cell staining, Hoechst 33342 dye …
DAPI - Wikipedia
DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine – thymine -rich regions in DNA. It is used extensively in fluorescence …
What Is DAPI and How Does It Stain DNA? - Biology Insights
DAPI is a fluorescent stain widely used in biological research to visualize DNA. Its strong affinity for DNA makes it a valuable tool for examining cell structures, identifying nuclei, and analyzing …
DAPI Staining – Protocol, Uses & Application Guide
Jul 16, 2025 · In this guide, you’ll learn the complete DAPI staining protocol, its uses, key precautions, and how to get consistent, reliable results every time. DAPI (4′,6-diamidino-2 …
Hoechst & DAPI Staining Protocols - Cell Staining with ...
Aug 8, 2024 · Both DAPI and Hoechst are minor-groove binding dyes with a preference for A/T-rich regions of DNA over G/C-rich DNA. The dyes have minimal fluorescence in solution, but …
Is DAPI flow cytometry still relevant in the age of high ...
Apr 24, 2025 · Since it can’t cross the membranes of live cells and only stains the DNA of dead or dying ones, it’s ideal for viability assays and cell cycle analysis. DAPI is simple, fast, …
DAPI Staining Guide for Clear Nuclear Imaging
Jun 10, 2025 · DAPI staining is a widely used method for visualising DNA in fixed or live cells, offering strong nuclear contrast in fluorescence microscopy. It binds specifically to A-T-rich …